Laser-Assisted Hatching

Assisted hatching helps implant the embryo in the uterus.

What is the assisted hatching technique?

Assisted hatching is an in vitro fertilization technique that uses a precise laser to make a small hole in the protective layer of the embryo (called “zona pellucida”) to make it possible to implant it in the uterus.

For the embryo to be implanted in the uterus, it must hatch out of this layer. Implantation often fails because the embryo is unable to break the zona pellucida on its own and remains trapped inside.

In what cases is assisted hatching used?

• Eggs with a thick zona pellucida.
• When thawed embryos are transferred (this area hardens during freezing).
• When embryos from women older than 37 are used, it is harder to break the zona pellucida in these embryos.
• Repeated implantation failures over various IVF cycles.
• Slow-developing embryos.
• Highly fragmented embryos.

ICSI (Precision Sperm Injection)

In vitro fertilization technique that facilitates the entry of the sperm into the egg.

What is the ICSI technique?

ICSI is the in vitro technique used to carry out fertilization in the laboratory. Using a micropipette facilitates the sperm’s entry into the egg.

When is it recommended?

It is especially recommended in cases of:

• Long-term sterility.
• Low semen quality.
• When an IVF cycle has been performed unsuccessfully.

 

PICSI (Functional Sperm Selection)

What is the PICSI technique?

This technique leverages the fact that the egg’s outer layer contains hyaluronan, a substance that binds specifically to receptors on mature sperm. By using hyaluronan, we attract mature sperm, effectively preselecting those best suited for fertilizing the egg. Unlike the ICSI method, which selects sperm based on shape and motility, PICSI allows us to focus on mature sperm. This approach closely resembles the natural selection process during conception.

When is it recommended?

 • Patients with borderline sperm analysis results
 • Patients who have undergone several unsuccessful cycles
 • Couples experiencing low fertilization rates or poor embryo quality

 

EndomTRIO (EMMA, ERA, ALICE)

The EndomTRIO package, consisting of EMMA, ERA, and ALICE tests, provides a thorough assessment of the endometrial environment. With these tests, doctors can recommend a tailored treatment plan to enhance the chances of a successful and healthy pregnancy.

What is the EMMA technique?

EMMA (Endometrial Microbiome Metagenomic Analysis) examines the microbiome of the endometrium, analyzing bacterial balance and identifying beneficial bacteria that support a successful pregnancy.

What is the ERA technique?

ERA (Endometrial Receptivity Analysis) determines the optimal timing for embryo implantation by assessing endometrial receptivity. Based on the results, we can schedule the embryo transfer for the time with the highest likelihood of pregnancy.

What is the ALICE technique?

ALICE (Analysis of Infectious Chronic Endometriosis) detects chronic endometrial inflammation and pathogenic bacteria often linked to chronic endometriosis. This condition can contribute to implantation failure or recurrent miscarriages.

Who is the EndomTRIO method suitable for?

The EndomTRIO method is recommended for:

• Couples who have experienced repeated implantation failure
• Patients with a history of recurrent miscarriages
• Individuals seeking a comprehensive assessment of endometrial health to enhance their chances of a successful pregnancy